Sonication-dependent electroporation of the erythromycin-producing bacterium Saccharopolyspora erythraea.
نویسندگان
چکیده
We report the development of an electrotransformation method applicable to all strains of Saccharopolyspora erythraea examined to date. Vegetatively grown mycelia were rendered electrocompetent by subjecting mycelial suspensions to ultrasound pulses. The protocol provides an alternative route for the introduction of DNA into filamentous microorganisms otherwise recalcitrant to transformation techniques.
منابع مشابه
Genome Sequence of Saccharopolyspora erythraea D, a Hyperproducer of Erythromycin
Saccharopolyspora erythraea is a Gram-positive bacterium that can produce antibiotics. However, this microorganism must often be genetically improved for higher production before it can be used in an industrial setting. Here, we report the whole-genome sequence of the industrial hyperproducer strong mutator Saccharopolyspora erythraea strain D.
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عنوان ژورنال:
- Applied and environmental microbiology
دوره 64 4 شماره
صفحات -
تاریخ انتشار 1998